Journal: International Journal of Biological Sciences

Article Title: Chronic Stress Induces Hepatic Steatosis via Brain-Hepatic Sympathetic Axis Mediated Catecholamine Resistance

doi: 10.7150/ijbs.126058

Figure Lengend Snippet: Blockade of adipose sympathetic nerve does not prevent chronic stress-induced impairment of hepatic lipid homeostasis. (A-D) FFA, leptin, adiponectin, resistin levels in serum, n = 10 per group. (E) Experimental scheme. (F) Body weight, n= 5:6. (G) Food intake, n = 5:6. (H-K) Serum ALT, AST, TG and TC concentrations, n = 5:6. (L-O) FFA, leptin, adiponectin, resistin levels in serum, n = 5:6. (P) Liver weight and liver anatomy, n = 5:6. (Q) Liver TG, n = 5:6. (R) H&E staining (scale bars, 20 μm) and Oil Red O staining (scale bars, 20 μm) of liver sections, n = 5:6. (S) Relative mRNA levels in liver, n = 5:6. The data are presented as mean ± SEM. * P < 0.05. Unpaired two-tailed Student's t -test and one-way ANOVA were used for statistical analysis.

Article Snippet: Serum leptin (E-EL-M3008), adiponectin (E-EL-M0002), resistin (E-EL-M3056), norepinephrine (NE, E-EL-0047), and hepatic cyclic adenosine monophosphate (cAMP, E-EL-0056) in liver extracts were measured using enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer's intructions (Elabscience, Wuhan, China).

Techniques: Staining, Two Tailed Test

Journal: bioRxiv

Article Title: High cholesterol increases myeloma tumour burden and promotes resistance to bortezomib

doi: 10.1101/2025.01.21.633916

Figure Lengend Snippet: ( A ) CD138+ve plasma cells from GSE47552 dataset were analysed using GEO2R and resistin expression is shown. ( B ) Whole bone marrow from bortezomib-responsive and bortezomib-resistant patients (GSE51940 dataset) was analysed using GEO2R and resistin expression is shown. ( C ) Resistin gene expression in 5TGM1, ST2 and ST2-derived BMAds (Adipo). ( D ) Relative resistin gene expression comparing undifferentiated ST2 (ST2) and ST2-derived BMAds (Adipo). ( E ) Resistin protein concentrations secreted by undifferentiated ST2 and ST2-derived BMAds (Adipo). ( F ) Relative resistin expression in BMAds compared to BMAds treated for 3 days with exogenous LDL. ( G ) Relative resistin expression in media from BMAds when treated with LDL or cocultured with 5TGM1 + LDL compared to BMAds on their own. ( H ) 5TGM1 myeloma cell line was cultured alone or cocultured with undifferentiated ST2 or BMAds and resistin gene expression was quantified. For 2 group comparison, two-tailed Student’s t-test was performed. *p< 0.05, **p < 0.01. For more than 2 groups one-way anova analysis was performed. If not otherwise indicated, *p< 0.05, **p < 0.01 & ***p < 0.001. Results are presented as mean ± SEM.

Article Snippet: Resistin concentrations were quantified in bone marrow and serum using mouse resistin ELISA kit (EMRETN, Invitrogen).

Techniques: Expressing, Derivative Assay, Cell Culture, Comparison, Two Tailed Test

Journal: bioRxiv

Article Title: High cholesterol increases myeloma tumour burden and promotes resistance to bortezomib

doi: 10.1101/2025.01.21.633916

Figure Lengend Snippet: ( A ) LDL/VLDL-C ratio in the bone marrow of C57BL/6 KaLwRij mice after 4 weeks of high cholesterol diet. Adipokine analysis in blood ( B ) and bone marrow plasma ( C ). Heat maps show relative expression of adipokines in a non-tumour mouse on cholesterol diet (CHOL), myeloma-bearing mouse (MM) and myeloma-bearing mouse on cholesterol diet (MM CHOL) compared to non-tumour control. ( D ) Relative resistin expression (expressed as fold-increase compared to control) in bone marrow plasma from non-tumour mice fed with control diet (CTR), cholesterol diet for 4 weeks (CHOL 4w) or cholesterol diet for 8 weeks (CHOL 8w). ( E ) Resistin expression in blood from control or cholesterol fed animals for 8 weeks. ( F ) Relative resistin expression (expressed as fold-increase compared to control) in bone marrow plasma in control non-tumour mice (CTR), myeloma-bearing mice (MM) and myeloma-bearing mice on cholesterol diet (MM CHOL) from inoculation. ( G ) Pearson correlation of IgG2bK paraprotein and resistin levels with 17 pairs of XY coming from control and tumour-bearing mice. For 2 group comparison, two-tailed Student’s t-test was performed. For more than 2 groups one-way anova analysis was performed. If not otherwise indicated, *p< 0.05 and ***p < 0.001 compared to control with no-tumour. Results are presented as mean ± SEM.

Article Snippet: Resistin concentrations were quantified in bone marrow and serum using mouse resistin ELISA kit (EMRETN, Invitrogen).

Techniques: Expressing, Control, Comparison, Two Tailed Test